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Immunofluorescence recoloring was utilized to restrict endogenous and illusory G alpha I proteins in LLC-PK1 cells. The recoloring of illusory proteins alpha-i.co">ALPHA LOGIN was distinguished as an expanded force of recoloring on films containing endogenous G alpha I proteins. Utilizing confocal microscopy and picture examination we restricted G alpha I-2 to a particular sub-space of the parallel layer of enraptured cells, the fanciful G alpha I-3/2 protein was then appeared to colocalize with endogenous G alpha I-2 in a similar sidelong plasma film area. The fanciful G alpha I-2/3 protein colocalized with endogenous G alpha I-3 on Golgi layers in LLC-PK1 cells.